DFF45/ICAD Antibody (3A11) Summary
| Immunogen |
DFFA (NP_004392.1 231 a.a. – 331 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TSSDVALASHILTALREKQAPELSLSSQDLELVTKEDPKALAVALNWDIKKTETVQEACERELALRLQQTQSLHSLRSISASKASPPGDLQNPKRARQDPT
|
| Specificity |
DFFA – DNA fragmentation factor, 45kDa, alpha polypeptide (3A11)
|
| Isotype |
IgG2a Kappa
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
DFFA
|
| Purity |
IgG purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
|
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
|
| Buffer |
PBS (pH 7.4)
|
| Preservative |
No Preservative
|
| Purity |
IgG purified
|
Notes
Quality control test: Antibody Reactive Against Recombinant Protein.
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for DFF45/ICAD Antibody (3A11)
- DFF1
- DFF1DNA fragmentation factor 45 kDa subunit
- DFF45
- DFF45DNA fragmentation factor subunit alpha
- DFF-45DNA fragmentation factor, 45 kD, alpha polypeptide
- DFFA
- DNA fragmentation factor, 45kDa, alpha polypeptide
- ICAD
- Inhibitor of CAD
Background
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]