Product: Bay 65-1943 (hydrochloride)
DEP-1/CD148 Antibody (261922) [Alexa Fluor® 405] Summary
| Specificity |
Detects human DEP‑1/CD148.
|
| Isotype |
IgG2b
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
PTPRJ
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.
|
Packaging, Storage & Formulations
| Storage |
Store the unopened product at 2 – 8 °C. Do not use past expiration date.
|
| Buffer |
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
|
| Preservative |
0.09% Sodium Azide
|
| Concentration |
Please see the vial label for concentration. If unlisted please contact technical services.
|
Notes
Alternate Names for DEP-1/CD148 Antibody (261922) [Alexa Fluor® 405]
- CD148 antigen
- CD148
- Density-enhanced phosphatase 1
- DEP1
- DEP-1
- DEP1EC 3.1.3.48
- HPTP eta
- HPTPeta
- HPTP-eta
- human density enhanced phosphatase-1
- protein tyrosine phosphatase, receptor type, J polypeptide
- protein tyrosine phosphatase, receptor type, J
- Protein-tyrosine phosphatase eta
- Protein-tyrosine phosphatase receptor type J
- PTPRJ
- receptor-type tyrosine-protein phosphatase eta
- R-PTP-ETA
- R-PTP-J
- SCC1
- susceptibility to colon cancer 1, mouse, homolog of
Background
Density Enhanced Protein Tyrosine Phosphatase (DEP-1), also known as CD148, HPTP-eta, and PTP receptor type J (PTPRJ), is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. A large (220 kilodalton) glycoprotein found at the cell surface, DEP-1 levels are increased with high cell density (1). DEP-1 phosphatase activity is enhanced by basement membrane proteins (2), suggesting it is involved in regulating cell adhesion and contact interactions. High levels of expression dampen PDGF (3), VEGF (4), and T-cell receptor (5) responses. DEP-1 is widely expressed in tissues, particularly ones forming epithelioid monolayers (6). In the immune system, DEP-1 is found on all cell lineages and is highest on granulocytes (7). Dep-1 is the mutated gene in the Susceptibility to Colon Cancer locus Scc1, which is altered in many human colorectal adenomas (8). Gene knockout mice lacking DEP-1 die at midgestation due to failures in cardiovascular development (9). DEP-1 dephosphorylates a variety of proteins, including the HGF (10), PDGF (11), and VEGF (4) receptors, and beta‑catenin (12). The recombinant protein is the intracellular region of DEP-1 containing the catalytic domain.