DDX1 Antibody Summary
| Immunogen |
The immunogen recognized by this antibody maps to a region between residues 500 and 550 of human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 1 using the numbering given in entry AAH12739.1 (GeneID 1653).
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| Predicted Species |
Mouse (100%), Rat (100%), Porcine (100%), Primate (100%), Guinea Pig (100%), Bovine (100%), Rabbit (100%), Fish (100%), Equine (100%), Canine (100%). Backed by our 100% Guarantee.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
DDX1
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows. Western Blot: 1:1,000 to 1:10,000. In some cases, the antibody may be diluted further than indicated.
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| Positive Control |
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Reactivity Notes
Human. Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat, Bovine, Dog, Horse, Rabbit, Guinea pig_10141, Pig, Panda, Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, Fruit fly_7230, West Indian ocean coelacanth, Duckbill platypus and Northern white-cheeked gibbon.
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
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| Buffer |
TBS and 0.1% BSA
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| Preservative |
0.09% Sodium Azide
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| Concentration |
0.2 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for DDX1 Antibody
- ATP-dependent RNA helicase DDX1
- DBP-RBDEAD box polypeptide 1
- DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
- DEAD box protein 1
- DEAD box protein retinoblastoma
- DEAD box-1
- DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1
- EC 3.6.1
- EC 3.6.1.10
- EC 3.6.4.13
- UKVH5d
Background
DEAD box proteins, a family of putative RNA helicases, are characterized by eight conserved amino acid motifs that are arranged in a core region as found in the prototypical member of the family, eIF-4A. The familys name is derived from the amino acid sequence Asp-Glu-Ala-Asp (DEAD) that is located within the ATP hydrolysis motif. DEAD box proteins have been implicated in translation initiation and RNA splicing, degradation, and stability. The DEAD box protein, DDX1, was identified in retinoblastoma cell lines, where it is coamplified with MYCN, a member of the MYC transcription factor family. DDX1 is expressed at elevated levels in fetal tissues of neural origin (retina and brain). It is both cytoplasmic and nuclear in DDX1-amplified neuroblastoma and retinoblastoma cell lines. However, it localizes specifically to the nucleus in non-amplified cell lines. Thus, DDX1 is thought to be involved in nuclear RNA metabolism. In addition, the decrease in mean disease-free survival of neuroblastoma patients exhibiting coamplification of DDX1 and MYCN as compared to those exhibiting MYCN amplification indicates a possible role for DDX1 in cancer formation in neural tissues.