Cytokeratin 8 Antibody (B22.1) Summary
Immunogen |
Cytoskeletal preparation from HeLa cells
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Localization |
Cytoplasmic
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Specificity |
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular (ring-like, perinuclear) from ductal (peripheral-predominant) carcinoma of the breast.
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Isotype |
IgG1 Kappa
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Clonality |
Monoclonal
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Host |
Mouse
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Gene |
KRT8
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Purity |
Protein A or G purified
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Applications/Dilutions
Dilutions |
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Application Notes |
Hu-chromosome location: 12q13.13
Immunohistochemistry-Paraffin 0.5 – 1.0 ug/ml for 30 minutes at RT Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 – 20 min followed by cooling at RT for 20 minutes. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Theoretical MW |
52.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Packaging, Storage & Formulations
Storage |
Store at 4C.
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Buffer |
10mM PBS and 0.05% BSA
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Preservative |
0.05% Sodium Azide
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Purity |
Protein A or G purified
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Alternate Names for Cytokeratin 8 Antibody (B22.1)
- CARD2
- CK8
- CK-8
- CYK8cytokeratin 8
- Cytokeratin 8
- Cytokeratin-8
- EndoA
- K2C8
- K8cytokeratin-8
- keratin 8
- keratin, type II cytoskeletal 8
- keratin-8
- KO
- KRT8
- Type-II keratin Kb8