Cytokeratin 8 Antibody (35betaH11) [DyLight 680]

Product: Homatropine (methylbromide)

Cytokeratin 8 Antibody (35betaH11) [DyLight 680] Summary

Immunogen
Clone 35betaH11 is a mouse monoclonal IgM antibody derived by fusion of NS-1 mouse myeloma cells with spleen cells from a Balb/c mouse immunized with a cytoskeletal preparation of the human hepotocellular carcinoma cell line Hep3B.
Marker
Epithelial Cell Marker
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
KRT8
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
35BetaH11 is reactive on frozen tissue sections after fixation in methanol. It is particularly effective on paraffin-embedded tissues after microwave pretreatment of the slides. In addition, 35BetaH11 is suitable for Immunocytochemistry, Immunoblotting. Optimal antibody dilution should be determined by titration; recommended range is 1:100 – 1:500 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. Although not tested this antibody may be useful in and Flow cytometry.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for Cytokeratin 8 Antibody (35betaH11) [DyLight 680]

  • CARD2
  • CK8
  • CK-8
  • CYK8cytokeratin 8
  • Cytokeratin 8
  • Cytokeratin-8
  • EndoA
  • K2C8
  • K8cytokeratin-8
  • keratin 8
  • keratin, type II cytoskeletal 8
  • keratin-8
  • KO
  • KRT8
  • Type-II keratin Kb8

PMID: 10323594