Cytokeratin 7 Antibody (KRT7/1198) – IHC-Prediluted Summary
| Immunogen |
Recombinant full-length human KRT7 protein
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| Localization |
Cytoplasmic
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| Marker |
Glandular and Transitional Epithelial Marker
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| Specificity |
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
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| Isotype |
IgG1
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| Clonality |
Monoclonal
|
| Host |
Mouse
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| Gene |
KRT7
|
| Purity |
Protein A or G purified
|
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Hu-chromosome location: 12q13.13 Immunohistochemistry-Paraffin 0.5 – 1.0 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 – 20 min followed by cooling at RT for 20 minutes. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
|
| Theoretical MW |
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C.
|
| Buffer |
10mM PBS and 0.05% BSA
|
| Preservative |
0.05% Sodium Azide
|
| Purity |
Protein A or G purified
|
Alternate Names for Cytokeratin 7 Antibody (KRT7/1198) – IHC-Prediluted
- CK7
- CK-7
- cytokeratin 7
- cytokeratin-7
- K2C7
- K7keratin, 55K type II cytoskeletal
- keratin 7
- keratin, type II cytoskeletal 7
- keratin-7
- MGC129731
- MGC3625
- sarcolectin
- SCLkeratin, simple epithelial type I, K7
- type II mesothelial keratin K7
- Type-II keratin Kb7