Cullin-7 Antibody Summary
| Immunogen |
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1679-1698 of Human Cul7 (C-terminus) coupled to KLH.
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| Specificity |
This product reacts with human and mouse Cullin 7. Cross reactivity with other human cullins may occur.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
CUL7
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| Purity |
Sterile filtered
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Applications/Dilutions
| Dilutions |
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| Application Notes |
The antibody immunoprecipitates in vitro translated protein and also immunoprecipitates protein from cell lysates. This antibody also co-immunoprecipitates associated proteins in overexpressed CuL7 systems. A 191.2 kDa band corresponding – human Cul7 is detected. Most cell lines expressing Cul7 can be used as a positive control. Researchers should determine optimal titers for other applications.
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| Publications |
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Reactivity Notes
Reactive in human
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
No buffer
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| Preservative |
0.01% Sodium Azide
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| Concentration |
85 mg/ml
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| Purity |
Sterile filtered
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Alternate Names for Cullin-7 Antibody
- CUL-7
- cullin 7
- cullin-7
- KIAA0076dJ20C7.5
Background
Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 7 is a component of E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Cullin 7 seems to be involved proteosomal degradation of proteins involved in endothelial proliferation and/or differentiation. Cullin 7 is part of a SCF-like complex consisting of CUL7, RBX1, SKP1, FBXW8 and GLMN isoform 1. Cullin 7 interacts with a complex of SKP1 and FBXW8, but not with SKP1 alone.