Connexin 40/GJA5 Antibody

Product: glucagon receptor antagonists-2

Connexin 40/GJA5 Antibody Summary

Immunogen
A synthetic peptide from 2d cytoplasmic domain of human 2d cytoplasmic domain of Connexin-40 (CX40, CXA5) conjugated to an immunogenic carrier protein was used as the antigen.
Specificity
Specific for Connexin-40.
Clonality
Polyclonal
Host
Rabbit
Gene
GJA5
Purity
Unpurified
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Applications/Dilutions

Dilutions
  • Western Blot 1:300-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin
Application Notes
Western Blot. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Although not tested this antibody may work in Immunohistochemistry-Paraffin/Frozen.

Reactivity Notes

Other species not yet tested but expected to work in rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Concentration
LYOPH
Purity
Unpurified
Reconstitution Instructions
Reconstitute with 0.1 ml sterilized water to desired concentration.

Alternate Names for Connexin 40/GJA5 Antibody

  • alpha 5, 40kD (connexin 40)
  • ATFB11
  • Connexin 40
  • connexin-40
  • CX40
  • gap junction alpha-5 protein
  • gap junction protein, alpha 5, 40kDa (connexin 40)
  • gap junction protein, alpha 5, 40kDa
  • GJA5
  • MGC11185

Background

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. A connexon is composed of a hexamer of connexins.

PMID: 8394704