Complement C8A/B/G Antibody (056B-373) Summary
| Immunogen |
Purified human C8
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| Specificity |
This antibody recognizes complement component 8 (C8), a 151 kDa member of the complement C6/C7/C8/C9 family, present in blood serum. C8 is a terminal component of the complement system, part of both the complement membrane attack complex (MAC), and important to MAC assembly. C8 binds to the C5b-7 complex, anchored to the membrane, creating C5b-8. C5b-8 binds C9 and catalyzes the polymerization of C9 molecules to form C5-b9 (MAC). C8 is thought to contain lipid binding sites, facilitating the insertion of MAC into the membrane. Defects in the alpha chain of C8 can result in complement C8 deficiency type I. Furthermore, C8 deficiencies can cause recurring bacterial infections, in particular from Neisseria meningitides.
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| Isotype |
IgG1
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
C8A
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| Purity |
Protein A purified
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Applications/Dilutions
| Dilutions |
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| Publications |
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Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
BBS (pH 8.2 – 8.4)
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| Preservative |
0.09% Sodium Azide
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| Concentration |
1.0 mg/ml
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| Purity |
Protein A purified
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Alternate Names for Complement C8A/B/G Antibody (056B-373)
- complement component 8, alpha polypeptide
- complement component C8 alpha chain
Background
C8 is synthesised in the liver, monocytes and fibroblasts and is a plasma protein consisting of three non identical polypeptide chains. The alpha-chain (64kDa) and gamma chain (22kDa) are disulphide linked, these associate non-covalently with the beta-chain (64kDa), to form the native C8 molecule with a molecular weight of 151kDa. Normal plasma concentration is 80mg/L. C8 is one of the terminal proteins of the complement pathways.C8 binds to the membrane bound C5b-7, C5b-8 combines with C9 to form the membrane attack complex (MAC). The MAC forms a hydrophilic, protein walled, transmembrane channel, formation of which leads to cell lysis and destruction.