Cofilin [p Tyr140, p Ser156] Antibody

Product: Angiotensin II 7-valine

Cofilin [p Tyr140, p Ser156] Antibody Summary

Immunogen
A synthetic peptide from the phospho Y140/S156 of human Cofilin-1 (Cofilin, non-muscle isoform) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat, mouse and monkey.
Modification
p Tyr140, p Ser156
Localization
subcellular location: nucleus matrix. cytoplasm, cytoskeleton. note: almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide. tissue specificity: widely distributed in various tissues.
Specificity
Cofilin-1.
Clonality
Polyclonal
Host
Sheep
Gene
CFL1
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • Western Blot 10-50 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 10-50 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
Positive Control
Cofilin Lysate (NBL1-09126)

Reactivity Notes

Mouse, Rat and Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
LYOPH
Purity
IgG purified
Reconstitution Instructions
Reconstitute with 0.5 ml sterilized water. Add glycerol (1:1) for additional stability.

Alternate Names for Cofilin [p Tyr140, p Ser156] Antibody

  • 18 kDa phosphoprotein
  • CFL
  • cofilin 1 (non-muscle)
  • Cofilin, non-muscle isoform
  • cofilin-1
  • p18

Background

Cofilin is a small phosphoinositide sensitive, actin binding protein capable of depolymerizing actin filaments in vitro. Under certain conditions, it fragments the filaments and accelerates actin subunit dissociation from their pointed (minus) ends. Cofilin binds stoichiometrically to monomeric G-actin and to actin protomers in filaments in an apparent pH-dependent, Ca2+ independent manner. Cofilin intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist. Cofilin is ubiquitous in tissues of eukaryotes and is especially abundant in neuronal tissues. It is essential for viability and is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, cytokinesis, and pathogen intracellular motility.

PMID: 10998544