Chk1 Antibody Summary
| Immunogen |
The antiserum was produced against synthesized non-phosphopeptide derived from human Chk1 around the phosphorylation site of serine 317 (S-S-SP-Q-P)
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
CHEK1
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Theoretical MW |
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Positive Control |
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Reactivity Notes
Human and Mouse.
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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| Buffer |
PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
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| Preservative |
0.02% Sodium Azide
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| Concentration |
1.0 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for Chk1 Antibody
- Checkpoint, S. pombe, homolog of, 1
- CHEK1
- CHK1 (checkpoint, S.pombe) homolog
- CHK1 checkpoint homolog (S. pombe)
- Chk1
- CHK1serine/threonine-protein kinase Chk1
- EC 2.7.11
- EC 2.7.11.1
Background
One mechanism by which checkpoints maintain the fidelity of cell cycle events is through blocking mitosis in response to unreplicated or damaged DNA. In most species this occurs through inhibiting activation of the cyclic dependent kinase Cdc2, which regulates entry into mitosis. Chk1, a kinase involved in the DNA damage checkpoint response can phosphorylate Cdc25C an activator of Cdc2. It is hypothesized that Chk1 induces serine 216 phosphorylation of Cdc25C and subsequent 14-3-3 binding negatively regulated Cdc25C, thus preventing it from activating Cdc2.