Chicken anti-Rat IgG (H+L) Secondary Antibody [HRP] (Pre-absorbed)

Product: PF-04691503

Chicken anti-Rat IgG (H L) Secondary Antibody [HRP] (Pre-absorbed) Summary

Immunogen
Purified Rat IgG (H&L)
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy (gamma) chains on rat IgG and light chains on all rat immunoglobulins. Cross Adsorbed: This antibody was pre-adsorbed to reduce cross-reactivity with proteins of non-related species. Minimal cross-reactivity with human, rabbit IgG serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Chicken
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:200-1:5000
  • ELISA 1:200-1:5000
  • Immunohistochemistry 1:500-1:5000
Application Notes
This conjugate is suitable for all immunoassay applications.

Reactivity Notes

Based on IEP, this antibody reacts with heavy (gamma) chains on rat IgG and light chains on all rat immunoglobulins.

Packaging, Storage & Formulations

Storage
Store at 4C. After reconstitution, dilute with 50% glycerol and store at -20C. Avoid repeated freeze-thaw cycles.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free.
Preservative
0.1% Kathon
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.55 ml deionized water.

Notes

Purity > 95% based on SDS-PAGE. Ig fraction was prepared using eggs obtained from healthy hens of US origin. After reconstituted, prepare fresh working dilution daily.

PMID: 9838034