Chicken anti-Mouse IgG (H+L) Secondary Antibody [FITC]

Product: Tolcapone

Chicken anti-Mouse IgG (H L) Secondary Antibody [FITC] Summary

Immunogen
Mouse whole molecule
Specificity
Mouse IgG (H and L)
Isotype
IgG
Clonality
Polyclonal
Host
Chicken
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 – 1:10000
  • Flow Cytometry 1:1:10 – 1:1000
  • Fluorophore-linked immunosorbent assay 1:10000 – 1:50000
  • Immunocytochemistry/Immunofluorescence 1:1000 – 1:5000
Publications
Read Publication using NB120-6810.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) – Immunoglobulin and Protease free
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Background

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10^11 variants.

PMID: 26033355