Chicken anti-Mouse IgG (H+L) Secondary Antibody [DyLight 650]

Product: NB-599 (Maleate)

Chicken anti-Mouse IgG (H L) Secondary Antibody [DyLight 650] Summary

Immunogen
Purified Mouse IgG (H&L)
Isotype
IgG
Clonality
Polyclonal
Host
Chicken
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:20 – 1:2000
  • Immunomicroscopy 1:20 – 1:2000
Application Notes
This conjugate is suitable for immunomicroscopy and flow cytometry.

Reactivity Notes

Mouse IgG, H/L Chains

Packaging, Storage & Formulations

Storage
Store at 4C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C. Avoid repeated freeze-thaw cycles.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.05% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with sterilized deionized water. Centrifuge to remove any particulates.

Notes

Affinity purified antibody is > 95% based on SDS-PAGE. Chicken Ig fraction was prepared using eggs from healthy hens of US origin. DyLight 650 (Ex = 652 nm; Em = 672 nm). After reconstituted, prepare fresh working dilution daily.

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

PMID: 9435912