Carbonic Anhydrase IX/CA9 Antibody (CA9/781) Summary
| Immunogen |
Recombinant human CA9 protein
|
| Localization |
Cell surface and cytoplasmic
|
| Marker |
Renal Cell Marker
|
| Specificity |
Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200). Carbonic Anhydrases (CAs) are members of a large family of zinc metallo-enzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. They show extensive diversity in distribution and in their subcellular localization. CA IX is specifically expressed in clear-cell renal carcinomas.
|
| Isotype |
IgG2b Kappa
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
CA9
|
| Purity |
Protein A or G purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Hu-chromosome location: 9p13.3
Immunohistochemistry-Paraffin 0.5 – 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 – 20 min followed by cooling at RT for 20 minutes. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Theoretical MW |
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C.
|
| Buffer |
10mM PBS and 0.05% BSA
|
| Preservative |
0.05% Sodium Azide
|
| Purity |
Protein A or G purified
|
Alternate Names for Carbonic Anhydrase IX/CA9 Antibody (CA9/781)
- CA9
- CA-IX
- CAIXcarbonic anhydrase 9
- Carbonate dehydratase IX
- Carbonic Anhydrase IX
- carbonic anhydrase IXpMW1
- carbonic dehydratase
- EC 4.2.1.1
- G250
- Membrane antigen MN
- MN
- MNRCC-associated antigen G250
- P54/58N
- RCC
- RCC-associated protein G250
- Renal cell carcinoma-associated antigen G250