Carbonic Anhydrase II/CA2 Antibody

Product: Procarbazine (Hydrochloride)

Carbonic Anhydrase II/CA2 Antibody Summary

Immunogen
Carbonic anhydrase II purified from bovine erythrocytes
Specificity
Carbonic Anhydrase II
Clonality
Polyclonal
Host
Rabbit
Gene
CA2
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 1:10-1:500
Positive Control
Carbonic Anhydrase II/CA2 Lysate (NBL1-08615)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
Preservative
0.01% Sodium Azide
Purity
IgG purified

Alternate Names for Carbonic Anhydrase II/CA2 Antibody

  • CA2
  • CAII
  • CA-IIEC 4.2.1.1
  • Car2
  • Carbonate dehydratase II
  • carbonic anhydrase 2
  • carbonic anhydrase B
  • Carbonic anhydrase C
  • Carbonic Anhydrase II
  • carbonic anhydrase IICAC
  • carbonic dehydratase

Background

Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20

PMID: 10428375

Carbonic Anhydrase II/CA2 Antibody

Product: AMD-071 (hydrochloride)

Carbonic Anhydrase II/CA2 Antibody Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Full length native Carbonic Anhydrase II (purified).
Localization
Cytoplasmic
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Bovine Erythrocytes].
Clonality
Polyclonal
Host
Rabbit
Gene
CA2
Purity
Ion exchange chromatography
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Test in a species/application not listed above to receive a full credit towards a future purchase.

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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • ELISA 1:20000-1:100000
  • Immunohistochemistry
Application Notes
This product has been assayed against 1.0 ug of Carbonic Anhydrase II [Bovine Erythrocytes] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] and TMBE as a substrate for 30 minutes at room temperature.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 9336012).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water to desired concentration.

Alternate Names for Carbonic Anhydrase II/CA2 Antibody

  • CA2
  • CAII
  • CA-IIEC 4.2.1.1
  • Car2
  • Carbonate dehydratase II
  • carbonic anhydrase 2
  • carbonic anhydrase B
  • Carbonic anhydrase C
  • Carbonic Anhydrase II
  • carbonic anhydrase IICAC
  • carbonic dehydratase

Background

Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29 kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20

PMID: 21440447