Calponin 1 Antibody Summary
| Immunogen |
Synthetic peptide C-KRQIFEPGLGMEH from an internal region (near C-terminus) of human CNN1 / Calponin (NP_001290.2).
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| Specificity |
Human CNN1 / Calponin.
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| Predicted Species |
Opossum (92%). Backed by our 100% Guarantee.
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| Clonality |
Polyclonal
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| Host |
Goat
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| Gene |
CNN1
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
IHC: NBP1-52399 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.
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Reactivity Notes
Gorilla, Gibbon, Marmoset. Predicted cross-reactivity based on sequence identity: Ferret (100%), Elephant (100%), Panda (100%), Lizard (92%), Chicken (85%).
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
TBS (pH 7.3) and 0.5% BSA
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| Preservative |
0.02% Sodium Azide
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| Concentration |
0.5 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for Calponin 1 Antibody
- Basic calponin
- Calponin 1
- calponin 1, basic, smooth muscle
- Calponin H1
- Calponin H1, smooth muscle
- calponins, basic
- CNN1
- Sm-Calp
- SMCC
- SMCCcalponin-1
Background
Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein thought to be involved in regulation of actomyosin as well as the regulation or modulation of contraction (1). Calponin binds filamentous actin (F-actin) through two distinct sites ABS1 and ABS2, with an affinity in the low micromolar range (2) Immunoreactivity for calponin, along with alpha-smooth muscle actin and smooth muscle myosin heavy chains, confirms the known neoplastic myoepithelial component of adenoid cystic carcinomas and epithelial-myoepithelial carcinomas. The consistently positive staining pattern in adenoid cystic carcinomas may be diagnostically useful in discriminating histologically similar but consistently negative polymorphous low-grade adenocarcinomas (3).