CEA Antibody (SPM584) – Azide and BSA Free

Product: AT7868 (dihydrochloride)

CEA Antibody (SPM584) – Azide and BSA Free Summary

Immunogen
Human colon carcinoma extract
Localization
Cytoplasmic and luminal surface
Specificity
This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CEACAM5
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • Flow Cytometry 0.5-1ug/ million cells
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5-1 ug/ml
  • CyTOF-ready
Application Notes
IHC:Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS with 0.05% BSA
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for CEA Antibody (SPM584) – Azide and BSA Free

  • Carcino Embryonic Antigen
  • Carcinoembryonic antigen

PMID: 16403947