CDC2/CDK1 Antibody (CDK1/873) Summary
| Immunogen |
Recombinant human CDK1 protein
|
| Localization |
Nuclear and Cytoplasmic
|
| Specificity |
Recognizes a 34kDa protein, which is identified as cyclin dependent kinase 1 (cdk1) or p34cdc2 protein kinase. cdk1 / p34cdc2 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated cdk1 / p34cdc2 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.
|
| Isotype |
IgG2a Kappa
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
CDC2, CDK1
|
| Purity |
Protein A or G purified
|
| Innovators Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovators Reward
|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Hu-chromosome location: 10q21.2
Immunohistochemistry-Paraffin 2 – 4 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 – 20 min followed by cooling at RT for 20 minutes. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Theoretical MW |
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Human, Monkey, Cow, and Mink. Does not react with mouse, Rat, Xenopus, drosophila, S. pombe, and S. cerevisiae. Others-not known.
Packaging, Storage & Formulations
| Storage |
Store at 4C.
|
| Buffer |
10mM PBS and 0.05% BSA
|
| Preservative |
0.05% Sodium Azide
|
| Concentration |
0.2 mg/ml
|
| Purity |
Protein A or G purified
|
Alternate Names for CDC2/CDK1 Antibody (CDK1/873)
- CDC2
- CDC28A
- CDC2MGC111195
- CDK1
- cell cycle controller CDC2
- Cell division control protein 2 homolog
- Cell division protein kinase 1
- cyclin-dependent kinase 1
- DKFZp686L20222
- EC 2.7.11.22
- EC 2.7.11.23
- G1 to S and G2 to M
- p34 protein kinase
- P34CDC2