CD99 Antibody (HO36-1.1) – IHC-Prediluted Summary
| Immunogen |
Purified E-rosette forming cells from human peripheral blood lymphocytes
|
| Localization |
Cell surface
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| Marker |
Ewings Sarcoma Marker
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| Specificity |
Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32kDa (p30/32).Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis.CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewings sarcoma cells and primitive peripheral neuroectodermal tumors. This MAb shows a very similar reactivity to other CD99 MAbs (e.g. O13, 12E7, or HBA-71) and is excellent for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
|
| Isotype |
IgM Kappa
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| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
CD99
|
| Purity |
Tissue culture supernatant
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Hu-chromosome location: Xp22.33 Molecular weight of antigen: 27-32kDa Immunohistochemistry-Paraffin 1:50 – 1:100 for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 – 20 min followed by cooling at RT for 20 minutes.
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Packaging, Storage & Formulations
| Storage |
Store at 4C.
|
| Buffer |
10mM PBS and 0.05% BSA
|
| Preservative |
0.05% Sodium Azide
|
| Purity |
Tissue culture supernatant
|
Alternate Names for CD99 Antibody (HO36-1.1) – IHC-Prediluted
- 12E7
- antigen identified by monoclonal antibodies 12E7, F21 and O13
- CD99 antigenY homolog
- CD99 molecule
- CD99
- E2 antigen
- HBA71
- MIC2 (monoclonal 12E7)
- MIC2
- MIC2Y
- MSK5X
- pilr-1
- PILR-L
- Protein MIC2
- surface antigen MIC2
- T-cell surface glycoprotein E2