CD8 alpha Antibody (HIT8a) Summary
| Immunogen |
The immunogen for this antibody was CD8.
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| Isotype |
IgG1 Kappa
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
CD8A
|
| Purity |
Protein A or G purified
|
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Applications/Dilutions
| Dilutions |
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| Application Notes |
The HIT8a antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
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Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
|
| Buffer |
Aqueous, may contain carrier protein/stabilizer.
|
| Preservative |
0.09% Sodium Azide
|
| Concentration |
0.5 mg/ml
|
| Purity |
Protein A or G purified
|
Alternate Names for CD8 alpha Antibody (HIT8a)
- CD8 antigen, alpha polypeptide (p32)
- CD8
- CD8a antigen
- CD8a molecule
- Leu2 T-lymphocyte antigen
- Leu2
- MAL
- OKT8 T-cell antigen
- p32
- RPA-T8 antibody flow
- RPA-T8 Antibody
- RPA-T8 CD8
- RPA-T8 Clone
- RPA-T8 Flow
- T cell co-receptor
- T8 T-cell antigen
- T-cell antigen Leu2
- T-cell surface glycoprotein CD8 alpha chain
- T-lymphocyte differentiation antigen T8/Leu-2
Background
The HIT8a monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alphabeta) or as a homodimer (CD8 alphaalpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.