CD45 Antibody (IBL-3/16)

Product: Erythromycin Ethylsuccinate

CD45 Antibody (IBL-3/16) Summary

Immunogen
Purified B cells from mouse lymph nodes
Specificity
Recognizes mouse CD45. The enhances the MHC Class II induced cluster formation of mouse B cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
PTPRC
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:50-1:100
  • Immunoprecipitation 1:10-1:500
Application Notes
The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended.

Reactivity Notes

Does not react with Rat or Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.1% Bovine Serum Albumin
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for CD45 Antibody (IBL-3/16)

  • B220
  • CD45 antigen
  • CD45
  • CD45R
  • EC 3.1.3.48
  • LCA
  • L-CA
  • LY5
  • protein tyrosine phosphatase, receptor type, C
  • PTPRC
  • receptor-type tyrosine-protein phosphatase C
  • T200 Glycoprotein
  • T200 leukocyte common antigen
  • T200receptor type, c polypeptide

Background

CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognizing a common epitope on all of the isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc.

PMID: 26074769