CD45RA Antibody (F8-11-13)

Product: Castanospermine

CD45RA Antibody (F8-11-13) Summary

Immunogen
Human T lymphocytes
Specificity
NB100-65789 recognizes the human CD45RA cell surface antigen. CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc. Clone F8-11-13 reacts with the high molecular weight form of the leucocyte common antigen (LCA) expressed by B lymphocytes and a subset of T lymphocytes. This product is routinely tested in flow cytometry on human peripheral blood lymphocytes.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PTPRC
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50 – 1:100
  • Immunohistochemistry 1:10 – 1:500
  • Immunohistochemistry-Frozen 1:10 – 1:500
  • Immunohistochemistry-Paraffin 1:200
Application Notes
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Positive Control
CD45 Lysate (NBL1-14981)

Reactivity Notes

Reacts with Human. Cross reacts with Rhesus Monkey.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD45RA Antibody (F8-11-13)

  • B220
  • CD45 antigen
  • CD45R
  • EC 3.1.3.48
  • L-CA
  • LY5
  • protein tyrosine phosphatase, receptor type, C
  • receptor-type tyrosine-protein phosphatase C
  • T200 glycoprotein
  • T200 leukocyte common antigen
  • T200receptor type, c polypeptide

Background

CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognizing a common epitope on all of the isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc.

PMID: 11111832