Product: PD 123320 (ditrifluoroacetate)
CD44 Antibody (SPM521) – IHC-Prediluted Summary
| Immunogen |
Stimulated human leukocytes
|
| Localization |
Cell surface
|
| Specificity |
Recognizes a cell surface glycoprotein of 80-95kDa (CD44) on lymphocytes, monocytes, and granulocytes. Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.
|
| Isotype |
IgG2a Kappa
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
CD44
|
| Purity |
Protein A or G purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Hu-chromosome location: 11p13 Molecular weight of antigen: 80-95kDa Functional Studies Order Ab without BSA & Azide; Immunohistochemistry-Paraffin 0.25 – 0.5 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 – 20 min followed by cooling at RT for 20 minutes.
|
Reactivity Notes
Human, Baboon, Green Monkey, Others-not tested.
Packaging, Storage & Formulations
| Storage |
Store at 4C.
|
| Buffer |
10mM PBS and 0.05% BSA
|
| Preservative |
0.05% Sodium Azide
|
| Purity |
Protein A or G purified
|
Alternate Names for CD44 Antibody (SPM521) – IHC-Prediluted
- CD44 antigen
- CD44 molecule (Indian blood group)
- CD44
- CD44R
- CDw44
- cell surface glycoprotein CD44
- chondroitin sulfate proteoglycan 8
- CSPG8
- ECMR-III
- epican
- Extracellular matrix receptor III
- GP90 lymphocyte homing/adhesion receptor
- HCAM
- HCELL
- hematopoietic cell E- and L-selectin ligand
- Heparan sulfate proteoglycan
- Hermes antigen
- homing function and Indian blood group system
- HUTCH-I
- Hyaluronate receptor
- IN
- LHR
- MC56
- MDU2
- MDU2CD44 antigen (homing function and Indian blood group system)
- MDU3
- MDU3CDW44
- MIC4
- MIC4MGC10468
- MUTCH-I
- Pgp1
- PGP-1
- PGP-I
- Phagocytic glycoprotein 1
- Phagocytic glycoprotein I