CD43/Sialophorin Antibody (R2/60) Summary
| Immunogen |
The immunogen for this antibody was CD43.
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| Isotype |
IgM
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| Clonality |
Monoclonal
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| Host |
Rat
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| Gene |
SPN
|
| Purity |
Protein A or G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
This R2/60 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
|
Reactivity Notes
This antibody is reactive to Mouse.
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
|
| Buffer |
Aqueous, may contain carrier protein/stabilizer.
|
| Preservative |
0.09% Sodium Azide
|
| Concentration |
0.5 mg/ml
|
| Purity |
Protein A or G purified
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Alternate Names for CD43/Sialophorin Antibody (R2/60)
- CD43 antigen
- CD43
- CD43)
- Galactoglycoprotein
- GALGP
- GPL115
- Leukocyte sialoglycoprotein
- Leukosialin
- LSN
- Ly-48
- sialophorin (gpL115, leukosialin, CD43)
- Sialophorin
- SPN
Background
The R2/60 monoclonal antibody reacts with mouse CD43, also known as leukosialin and sialophorin. CD43 has glycoforms of approximately 115 and 130 kDa, and is expressed by an early B cell subset in BM, thymocytes and mature T cells, NK cells, myeloid lineage cells and platelets. CD43 is reported to bind to CD54 and play a role in adhesion and B cell survival. While the S7 and 1B11 monoclonal antibody clones detect only certain isoforms of CD43, data suggests that R2/60 detects the expression of both isoforms. It has been demonstrated that R2/60 stains the majority of CD4+ and CD8+ splenocytes, as well as a significant population of CD19+ splenocytes. However, the clones S7 and 1B11 stain only CD4+ and CD8+, or CD19+ splenocytes, respectively. This suggests that S7 and 1B11 recognize distinct epitopes which are differentially expressed by splenic B and T cells, whereas R2/60 recognizes an epitope shared by both isoforms of CD43.