Product: Pemetrexed (disodium hemipenta hydrate)
CD3 epsilon Antibody (APA1/1) Summary
Immunogen |
Purified human CD3 proteins isolated from thymus
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Specificity |
The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.
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Isotype |
IgG1
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Clonality |
Monoclonal
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Host |
Mouse
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Gene |
CD3E
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Purity |
Protein A purified
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Applications/Dilutions
Dilutions |
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Application Notes |
Flow Cytometry: Sample preparation – At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS + 0.1% BSA for 20 min. on ice. Wash with PBS and fix with 2% formaldehyde, 30 min on ice. Wash with PBS and incubate in PBS + 0,1% saponine, 5 min. RT. Incubate the cells in PBS + 1% BSA + 0.03% saponine, 15 min. on ice. Incubate with fluorescence-labeled APA1/1 antibody (1-2 ug/ml) in PBS + 1% BSA + 0.03% saponine in dark, 20 min. RT. Wash with PBS + 1% BSA + 0.03% saponine, resuspend in PBS. Immunocytochemistry: Fixed and permeabilised cells. The antibody can distinguish TCR-stimulated from non-stimulated cells.
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Reactivity Notes
Packaging, Storage & Formulations
Storage |
Store at 4C. Do not freeze.
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Buffer |
PBS (pH 7.4)
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Preservative |
15mM Sodium Azide
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Concentration |
1.0 mg/ml
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Purity |
Protein A purified
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Alternate Names for CD3 epsilon Antibody (APA1/1)
- CD3 epsilon
- CD3e antigen
- CD3e antigen, epsilon polypeptide (TiT3 complex)
- CD3e molecule, epsilon (CD3-TCR complex)
- CD3e
- CD3-epsilon
- FLJ18683
- T3E
- T-cell antigen receptor complex, epsilon subunit of T3
- T-cell surface antigen T3/Leu-4 epsilon chain
- T-cell surface glycoprotein CD3 epsilon chain
- TCRE