CD34 Antibody (4H11[APG]) [Alexa Fluor® 405]

Product: Azimilide

CD34 Antibody (4H11[APG]) [Alexa Fluor® 405] Summary

Immunogen
Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.
Localization
Membrane; Single-pass type I membrane protein.
Marker
Hematopoietic Stem Cell Marker
Specificity
The antibody 4H11[APG] reacts with Class III epitope on CD34 (Mucosialin), a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. The antibody 4H11[APG] completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line. HLDA VI; WS Code M MA58
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD34
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Flow (Cell Surface)
Application Notes
This product has been validated for use in Flow Cytometry, Western Blot, Immunohistochemistry-Paraffin and Immunocytochemistry. Western blot sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Recommended positive control for IHC-P: Placenta endothelium.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for CD34 Antibody (4H11[APG]) [Alexa Fluor® 405]

  • CD34 antigenhematopoietic progenitor cell antigen CD34
  • CD34 molecule
  • CD34
  • HPCA1

PMID: 26812940