CD163 Antibody (GHI/61) Summary
| Immunogen |
The immunogen for this antibody was CD163.
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| Marker |
Histiocytic Marker
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| Isotype |
IgG1 Kappa
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
CD163
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| Purity |
Protein A or G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
This GHI/61 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
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| Publications |
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Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
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| Buffer |
Aqueous, may contain carrier protein/stabilizer.
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| Preservative |
0.09% Sodium Azide
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| Purity |
Protein A or G purified
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Alternate Names for CD163 Antibody (GHI/61)
- CD163 antigenscavenger receptor cysteine-rich type 1 protein M130
- CD163 molecule
- CD163
- GHI/61
- HbSR
- Hemoglobin scavenger receptor
- M130
- M130macrophage-associated antigen
- MM130
- RM3/1
Background
The GHI/61 monoclonal antibody reacts with human CD163 (Haemoglobin Scavenger Receptor, M130), a member of the macrophage scavenger receptor family. CD163 is expressed by tissue macrophages, circulating blood monocytes, and some reports suggest that it may also be expressed by haematopoietic progenitor cells. CD163 functions as a high affinity scavenger receptor for the complex of haemoglobin and haptoglobin which forms to clear haemoglobin from the blood. The high affinity for CD163 is specific for the haemoglobin-haptoglobin complex, whereas haemoglobin or haptoglobin display no to low affinity toward CD163. Upon ligand binding, CD163 initiates signal transduction which leads to the production of IL-6 and IL-10. Furthermore, it has been demonstrated that cytokines such as IL-6 and IL-10 can induce expression of CD163, whereas proinflammatory stimuli, such as LPS or TNFalpha, can cause shedding of a soluble form of CD163, whose function is unclear.