CD117/c-kit Antibody (104D2) Summary
| Immunogen |
Unfortunately, this information is considered to be commercially sensitive
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| Localization |
Type I membrane protein.
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| Marker |
Hematopoietic Stem Cell Marker
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| Specificity |
This recognises the human c-Kit. c-Kit is expressed by some haematopoietic stem cells (approx. 70% of CD34 +ve cells in normal bone marrow are c-Kit +ve), by some acute myeloid leukaemias and by mast cells.This is non-neutralising.
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| Isotype |
IgG1
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
KIT
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| Purity |
Protein A or G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Flow Cytometry Use at a dilution of 1/2 – 1/20. Use 10ul of the suggested working dilution to label 106 cells in 100ul.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24664884)
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| Publications |
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Reactivity Notes
Cross reacts with Cynomolgus Monkey. Mouse reactivity reported in scientific literature (PMID: 24664884) Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
Phosphate buffered saline
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| Preservative |
0.09% Sodium Azide
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| Concentration |
0.5 mg/ml
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| Purity |
Protein A or G purified
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Alternate Names for CD117/c-kit Antibody (104D2)
- CD117 antigen
- CD117
- ckit
- c-kit
- EC 2.7.10
- EC 2.7.10.1
- KIT
- PBT
- piebald trait
- Proto-oncogene c-Kit
- proto-oncogene tyrosine-protein kinase Kit
- SCF R
- SCFRmast/stem cell growth factor receptor
- soluble KIT variant 1
- Tyrosine-protein kinase Kit
- v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
- v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein