CD117/c-kit Antibody (104D2)

Product: CP21R7

CD117/c-kit Antibody (104D2) Summary

Immunogen
Unfortunately, this information is considered to be commercially sensitive
Localization
Type I membrane protein.
Marker
Hematopoietic Stem Cell Marker
Specificity
This recognises the human c-Kit. c-Kit is expressed by some haematopoietic stem cells (approx. 70% of CD34 +ve cells in normal bone marrow are c-Kit +ve), by some acute myeloid leukaemias and by mast cells.This is non-neutralising.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KIT
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:2-1:20
  • Immunocytochemistry/Immunofluorescence
Application Notes
Flow Cytometry Use at a dilution of 1/2 – 1/20. Use 10ul of the suggested working dilution to label 106 cells in 100ul.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24664884)
Publications
Read Publication using
NB600-765 in the following applications:

  • FLOW
    1 publication
  • ICC/IF
    1 publication

Reactivity Notes

Cross reacts with Cynomolgus Monkey. Mouse reactivity reported in scientific literature (PMID: 24664884) Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CD117/c-kit Antibody (104D2)

  • CD117 antigen
  • CD117
  • ckit
  • c-kit
  • EC 2.7.10
  • EC 2.7.10.1
  • KIT
  • PBT
  • piebald trait
  • Proto-oncogene c-Kit
  • proto-oncogene tyrosine-protein kinase Kit
  • SCF R
  • SCFRmast/stem cell growth factor receptor
  • soluble KIT variant 1
  • Tyrosine-protein kinase Kit
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

PMID: 18202014