Bub1 Antibody Summary
| Immunogen |
Antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human Bub1. The immunogen is located within amino acids 40 – 90 of Bub1.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
BUB1
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Control Peptide |
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Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
PBS
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| Preservative |
0.02% Sodium Azide
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| Concentration |
1 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for Bub1 Antibody
- BUB1 budding uninhibited by benzimidazoles 1 homolog
- Bub1
- BUB1Amitotic checkpoint serine/threonine-protein kinase BUB1
- BUB1L
- budding uninhibited by benzimidazoles 1 (yeast homolog)
- budding uninhibited by benzimidazoles 1 homolog (yeast)
- EC 2.7.11.1
- hBUB1mitotic spindle checkpoint kinase
- putative serine/threonine-protein kinase
Background
Bub1 was initially identified as the mammalian homolog to the yeast protein through a cDNA screen. Bub1 encodes a kinase involved in the formation of the spindle checkpoint, an important mechanism that ensures high fidelity mitotic chromosome segregation. It is thought to be required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshim protein and metaphase congression. Bub1 functions by phosphorylating cdc20, a member of the mitotic checkpoint complex and activating the spindle checkpoint. A related protein kinase Bub3 interacts with Bub1 and targets it to kinetochores prior to chromosome alignment. Mutations in bub1 have been associated with aneuploidy and several forms of cancer.