Bu-1a/b Antibody (21-1A4) [Biotin] Summary
| Immunogen |
Bursa cells from one-day-old H.B15 (Bu-1a/b) strain chickens
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| Specificity |
Chicken Bu-1a/b alloantigen, Mr 70 kDa
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| Isotype |
IgG1 Kappa
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| Clonality |
Monoclonal
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| Host |
Mouse
|
| Purity |
Protein A or G purified
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Applications/Dilutions
| Dilutions |
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| Readout System |
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Reactivity Notes
Chicken
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
|
| Buffer |
PBS
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| Preservative |
0.05% Sodium Azide
|
| Purity |
Protein A or G purified
|
Notes
Protect conjugated forms from light.
Background
Chicken Bu-1a, a product of the Bu-1a allele, is expressed on the cell surface as a disulfide-linked homodimer. In 10-week-old H.B15 and H.B2 chickens, Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1a does not exhibit allelic exclusion. The monoclonal antibody 21-1A4 does not react with cells from CHA and H.B15 strains by immunofluorescence. (1-2)