Bu-1a/b Antibody (21-1A4)

Product: Lidocaine (hydrochloride)

Bu-1a/b Antibody (21-1A4) Summary

Immunogen
Bursa cells from one-day-old H.B15 (Bu-1a/b) strain chickens
Specificity
Chicken Bu-1a/b alloantigen, Mr 70 kDa
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry <= 1 ug/10^6 cells
  • Immunohistochemistry 1:10 – 1:500
  • Immunohistochemistry-Frozen 1:10 – 1:500
  • Immunoprecipitation 1:10 – 1:500
  • Immunocytochemistry

Reactivity Notes

Chicken

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.1M BBS (pH 8.2)
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Background

Chicken Bu-1a/b, a product of the Bu-1a allele, is expressed on the cell surface as a disulfide-linked homodimer. In 10-week-old H.B15 and H.B2 chickens, Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1a does not exhibit allelic exclusion. The monoclonal antibody 21-1A4 does not react with cells from CHA and H.B15 strains by immunofluorescence. (1-2)

PMID: 25723814