Blood Group Lewis b Antibody (2-25LE) Summary
| Immunogen |
Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglios classification).
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| Specificity |
The antibody 2-25LE reacts with Lewisb blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals.
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| Isotype |
IgG1
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
FUT3
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| Purity |
Protein A purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Purity is > 95% (by SDS-PAGE). Heat mediated antigen retrieval (sodium citrate); mAb incubation 1 hour / RT; detection DAB
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| Publications |
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Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
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| Buffer |
PBS (approx. pH 7.4)
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| Preservative |
15mM Sodium Azide
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| Concentration |
1.0 mg/ml
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| Purity |
Protein A purified
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Alternate Names for Blood Group Lewis b Antibody (2-25LE)
- alpha-(1,3/1,4)-fucosyltransferase
- Blood group Lewis alpha-4-fucosyltransferase
- CD174
- EC 2.4.1
- EC 2.4.1.65
- FT3B
- fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group)
- Fucosyltransferase 3
- Fucosyltransferase III
- FucT-III
- galactoside 3(4)-L-fucosyltransferase
- LEfucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood groupincluded)
- Les
- Lewis FT
- MGC131739
Background
The antibody 2-25LE reacts with Lewisb blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals.