Biotin (Vitamin B7 or Vitamin H) Antibody (SPM375) [Biotin] Summary
Immunogen |
Biotinylated sheep immunoglobulin
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Localization |
Depends on the target
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Specificity |
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
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Isotype |
IgG1
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Clonality |
Monoclonal
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Host |
Mouse
|
Purity |
Protein G purified
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Applications/Dilutions
Dilutions |
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Application Notes |
Immunohistochemistry (No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.)
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Theoretical MW |
244 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Readout System |
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Packaging, Storage & Formulations
Storage |
Store at 4C in the dark.
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Buffer |
PBS
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Preservative |
0.05% Sodium Azide
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Purity |
Protein G purified
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