Product: Clindamycin (hydrochloride)
Bcl-2 Antibody (BCL2/782) – Azide and BSA Free Summary
| Immunogen |
Recombinant full-length human bcl-2 protein
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| Localization |
Outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes.
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| Marker |
Apoptosis & Follicular Lymphoma Marker
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| Specificity |
This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
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| Isotype |
IgG1 Kappa
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
BCL2
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| Purity |
Protein A or G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Hu-chromosome location: 18q21.33 Immunohistochemistry-Paraffin 0.5 – 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10 – 20 min followed by cooling at RT for 20 minutes. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
|
| Theoretical MW |
25.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
PBS
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| Preservative |
No Preservative
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| Concentration |
1.0 mg/ml
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| Purity |
Protein A or G purified
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Alternate Names for Bcl-2 Antibody (BCL2/782) – Azide and BSA Free
- apoptosis regulator Bcl-2
- B-cell CLL/lymphoma 2
- Bcl2
- Bcl-2