Bcl-2 Antibody (118712) [Unconjugated] Summary
| Immunogen |
E. coli-derived recombinant human Bcl-2 minus the carboxyl-terminal mitochondrial targeting sequence
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| Specificity |
Detects human Bcl-2 Minus C-Terminus. Neutralizes recombinant human (rh) Bcl-2 bioactivity but not rhBcl-w or rhBcl-x bioactivity.
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| Source |
N/A
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| Isotype |
IgG1
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
BCL2
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Applications/Dilutions
| Dilutions |
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Packaging, Storage & Formulations
| Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
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| Preservative |
No Preservative
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| Reconstitution Instructions |
Reconstitute at 0.5 mg/mL in sterile PBS.
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Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Bcl-2 Antibody (118712) [Unconjugated]
- apoptosis regulator Bcl-2
- B-cell CLL/lymphoma 2
- Bcl2
- Bcl-2
Background
Bcl-2 is a member of a family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-2 is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-2 is present on the outer mitochondrial membrane and is also found on other membranes in some cell types. Natural Bcl-2 contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-2, missing the mitochondrial targeting sequence, maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-2 appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.