Bcl-2 Antibody (118712) [Unconjugated]

Product: BMS-708164

Bcl-2 Antibody (118712) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Bcl-2 minus the carboxyl-terminal mitochondrial targeting sequence
Specificity
Detects human Bcl-2 Minus C-Terminus. Neutralizes recombinant human (rh) Bcl-2 bioactivity but not rhBcl-w or rhBcl-x bioactivity.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BCL2
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Applications/Dilutions

Dilutions
  • Neutralization

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Bcl-2 Antibody (118712) [Unconjugated]

  • apoptosis regulator Bcl-2
  • B-cell CLL/lymphoma 2
  • Bcl2
  • Bcl-2

Background

Bcl-2 is a member of a family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-2 is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-2 is present on the outer mitochondrial membrane and is also found on other membranes in some cell types. Natural Bcl-2 contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-2, missing the mitochondrial targeting sequence, maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-2 appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.

 

PMID: 24178759