Product: Carvedilol metabolite 6-Hydroxyphenyl Carvedilol
Band 3 Antibody (BIII 136) Summary
| Immunogen |
Glycophorin B from human erythrocytes.
|
| Epitope |
The antibody recognizes an epitope located in the cytoplasmic pole of the Band 3 molecule. This epitope is within approximately 20 kD from the N-terminal end.
|
| Localization |
Cell Membrane; multi-pass membrane protein
|
| Specificity |
The antibody recognizes an epitope located in the cytoplasmic pole of the Band 3 molecule. This epitope is within approximately 20 kDa from the N-terminal end. This recognizes Band 3 protein (90-100 kD) and several lower molecular mass peptides migrating in SDS-PAGE gels in the regions of 60, 40 and 20 kD.
|
| Isotype |
IgG2a
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
SLC4A1
|
| Purity |
Unpurified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Immunofluorescent staining of fixed human erythrocytes. WB: Use at an assay dependent dilution. The antibody titer (1:5,000) was determined by indirect immunoblotting using an extract of human erythrocyte ghosts. This antibody recognizes Band 3 protein (90-100 kD) and several lower molecular mass peptides migrating in SDS-PAGE gels in the regions of 60, 40 and 20 kD. Digestion and aggregation of the Band 3 protein results in its detection with multiple molecular weight proteins.Since the epitope is not located at the erythrocyte surface, the antibody does not agglutinate red blood cells and its binding to the cell surface cannot be detected by an indirect agglutination assay.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
|
Reactivity Notes
Not yet tested in other species.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
Ascites
|
| Preservative |
15mM Sodium Azide
|
| Purity |
Unpurified
|
Alternate Names for Band 3 Antibody (BIII 136)
- AE 1
- AE1MGC126619
- Anion exchange protein 1
- Anion exchanger 1
- band 3 anion transport protein
- BND3
- CD233 antigen
- CD233
- DI
- EMPB3
- EPB3MGC126623
- erythrocyte membrane protein band 3
- erythroid anion exchange protein
- FR
- Froese blood group
- MGC116750
- MGC116753
- RTA1A
- Solute carrier family 4 member 1
- solute carrier family 4, anion exchanger, member 1 (erythrocyte membraneprotein band 3, Diego blood group)
- solute carrier family 4, anion exchanger, number 1
- SW
- Swann blood group
- Waldner blood group
- WD
- WD1
- WR
- Wright blood group