BRN3B/POU4F2 Antibody

Product: BMS-378806

BRN3B/POU4F2 Antibody Summary

Immunogen
Peptide with sequence HGLPSHMGCMSDVD corresponding to internal region according to NP_004566.2.
Specificity
This antibody is not expected to cross-react to the similar POU4F3 (GeneID 5459)
Clonality
Polyclonal
Host
Goat
Gene
POU4F2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100 – 1:2000
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Preliminary experiments gave an approx. 70 kDa band in rat retina lysates after 0.03 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 53.2 kDa band according to NP_599182.1. The 70 kDa band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered to be “Innovators Grade”. Innovators Grade antibodies have been validated for limited uses and require additional characterization for most new species/applications. These antibodies are directed against antigens that have limited or no commercial antibodies available for them, or for which there are limited expression data available to us. In an effort to encourage customer feedback, Novus has made these antibodies available through our Innovators Reward program. Under this program, Novus will provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal value in exchange for your new data. Please contact us at [email protected] for more details.

Alternate Names for BRN3B/POU4F2 Antibody

  • POU domain, class 4, transcription factor 2
  • BRN3.2
  • BRN3B
  • Brn-3b
  • POU class 4 homeobox 2

PMID: 16641247