Product: Galanthamine (hydrobromide)
Annexin V Apoptosis Kit [Fluorescein] Summary
| Description |
Please read the Instructions for Use prior to using this kit. Apoptosis is oftendefined by morphological criterion accompanied by biochemical analysis.Apoptosis is characterized by a number of intracellular phenomena such asmembrane blebbing, chromatin condensation and nuclear DNA fragmentation.Annexin V allows identification of cell surface changes that occur early during theapoptotic process either by flow cytometry or in situ detection. The Annexin V-FITCconjugate facilitates rapid fluorometric quantitation of apoptotic cells. The AnnexinV-Biotin conjugate offers flexibility in labeling by allowing the use of streptavidinconjugatedfluorophores other than FITC for detection.Early in the apoptosis process, phosphatidylserine (PS) becomes exposed onthe cell surface by flipping from the inner to outer leaflet of the cytoplasmic membrane(Figure 1). This event is thought to be important for macrophage recognitionof cells undergoing apoptosis, thus allowing the cells to be rapidly phagocytosed.The binding of Annexin V to phosphatidylserine is calcium-dependent, reversible,but very tight, with a Kd of approximately 5 x 10^-10 M. At low PS concentration, abinding ratio of 8 annexins to one PS has been reported. These features makeAnnexin V conjugates ideal for identifying membrane changes associated withapoptosis, by using either flow cytometric or in situ labeling methods.
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| Kit Type |
Apoptosis Kit
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| Gene |
ANXA5
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Packaging, Storage & Formulations
| Storage |
Store the unopened product at 2 – 8° C. Protect from light. Do not use past expiration date.
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Kit Components
| Components |
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Alternate Names for Annexin V Apoptosis Kit [Fluorescein]
- Anchorin CII
- annexin A5
- Annexin V
- annexin-5
- ANX5
- ANXA5
- ANXV
- Calphobindin I
- Endonexin II
- ENX2CBP-I
- Lipocortin V
- PAP-I
- Placental anticoagulant protein 4
- Placental anticoagulant protein I
- PP4
- Thromboplastin inhibitor
- VAC-alpha
- Vascular anticoagulant-alpha
Background
Annexin V is a member of a calcium and phospholipid binding family of proteins with vascular anticoagulant activity. It is also known as PP4, PAP I, CPB-I, CaBP33, VACa, anchorin CII, lipocortin-V, endonexin II, and thromboplastin inhibitor.