AlphaB Crystallin/CRYAB [p Ser45] Antibody Summary
| Immunogen |
Synthetic phopshopeptide corresponding to residues L(44) S(p) P F Y L R P P S F(54) C of human alpha-B Crystallin.
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| Modification |
p Ser45
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| Localization |
Cytoplasmic
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| Specificity |
This does not detect the unphosphorylated form of the protein.
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| Isotype |
IgG
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
CRYAB
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
WB: Detects an approx. 20 kDa representing recombinant bovine phospho-alpha-B crystallin (Ser45). IF: Staining of phospho-alpha-B crystallin (Ser45) in U373 MG cells results in cytoplasmic staining.
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| Positive Control |
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Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
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| Buffer |
PBS
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| Preservative |
0.05% Sodium Azide
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| Concentration |
LYOPH
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| Purity |
Immunogen affinity purified
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| Reconstitution Instructions |
Reconstitute to desired concentration.
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Alternate Names for AlphaB Crystallin/CRYAB [p Ser45] Antibody
- alpha B crystallin
- alpha(B)-crystallin
- AlphaB Crystallin
- alpha-crystallin B chain
- CRYA2
- CRYA2alpha crystallin B chain
- CRYAB
- crystallin, alpha B
- CTPP2
- Heat shock protein beta-5
- heat-shock 20 kD like-protein
- HSPB5
- Renal carcinoma antigen NY-REN-27
- Rosenthal fiber component
Background
Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products–alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexanders disease where it accumulates in brain cells of those afflicted.