AlphaA Crystallin/CRYAA Antibody (1H3.B8)

Product: XMU-MP-2

AlphaA Crystallin/CRYAA Antibody (1H3.B8) Summary

Immunogen
Native Alpha Crystallin.
Localization
Cytoplasm, Nucleus
Specificity
Detects approx 20kDa. Does not cross-react with aB-crystallin, Beta L-crystallin, BH- crystallin, gamma-crystallin, HSP25, HSP27 or HSP47 proteins.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CRYAA
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
Application Notes
0.5 ug/ml was sufficient for detection of 100 ng purified alphaA crystalline by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.Alpha A Crystallin Antibody.

Reactivity Notes

Based on homology, Its predicted to detect Mouse, Rat and Bovine.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for AlphaA Crystallin/CRYAA Antibody (1H3.B8)

  • AlphaA Crystallin
  • CRYA1
  • CRYA1crystallin, alpha-1
  • CRYAA
  • crystallin, alpha A
  • Heat shock protein beta-4
  • HSPB4
  • HSPB4alpha-crystallin A chain
  • human alphaA-crystallin (CRYA1), 10HspB4

Background

0.5ug/ml was sufficient for detection of 100ng purified alphaA crystalline by colorimetric immunoblot analysis using Goat Anti-Mouse IgG: HRP as the secondary.

PMID: 12702569