Aldolase A Antibody

Product: VT-465 (R enantiomer)

Aldolase A Antibody Summary

Description
This antibody was prepared from monospecific antiserum by a delipidation and defibrination.
Immunogen
Aldolase [Rabbit Muscle]
Clonality
Polyclonal
Host
Goat
Gene
ALDOA
Purity
Delipidation and Defibrination
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Applications/Dilutions

Dilutions
  • Western Blot 1:500 – 1:5000
  • ELISA 1:5000 – 1:20000
  • Immunoprecipitation 1:100
Application Notes
This product has been assayed against 1.0 ug of Aldolase [Rabbit Muscle] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code and (ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 of the reconstitution concentration is suggested for this product. Use approximately 5 ul of antibody to immunoprecipitate 50 ul of protein lysate.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
90 mg/ml
Purity
Delipidation and Defibrination
Reconstitution Instructions
Reconstitute with 2.0 ml deionized water to desired concentration.

Alternate Names for Aldolase A Antibody

  • ALDA
  • aldolase A, fructose-bisphosphate
  • EC 4.1.2.13
  • fructose-1,6-bisphosphate triosephosphate-lyase
  • fructose-bisphosphate aldolase A
  • GSD12
  • Lung cancer antigen NY-LU-1
  • MGC10942
  • MGC17716
  • MGC17767
  • Muscle-type aldolase

PMID: 15782150