Aldo-keto Reductase 1C1/AKR1C1 Antibody (6D10) Summary
| Immunogen |
Recombinant human AKR1C1 (1-323aa) purified from E. coli
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| Specificity |
Anti-human AKR1C1 mAb, clone 6D10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human AKR1C1 protein.
|
| Isotype |
IgG1 Kappa
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| Clonality |
Monoclonal
|
| Host |
Mouse
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| Gene |
AKR1C1
|
| Purity |
Protein G purified
|
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Applications/Dilutions
| Dilutions |
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| Application Notes |
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000. Recommended starting dilution is 1:1000
|
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
PBS (pH 7.4) and 10% Glycerol
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| Preservative |
0.02% Sodium Azide
|
| Concentration |
1 mg/ml
|
| Purity |
Protein G purified
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Alternate Names for Aldo-keto Reductase 1C1/AKR1C1 Antibody (6D10)
- 20 alpha-hydroxysteroid dehydrogenase
- 20-ALPHA-HSD
- 20-alpha-hydroxysteroid dehydrogenase
- 2-ALPHA-HSD
- AKR1C1
- Aldo-keto Reductase 1C1
- aldo-keto reductase family 1 member C1
- aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1; 20-alpha(3-alpha)-hydroxysteroid dehydrogenase)
- AldoketoReductase 1C1
- C9
- Chlordecone reductase homolog HAKRC
- DD1/DD2
- DD1MGC8954
- DDH1
- DDHH-37
- dihydrodiol dehydrogenase 1
- Dihydrodiol dehydrogenase 1/2
- dihydrodiol dehydrogenase isoform DD1
- EC 1.1.1
- EC 1.1.1.-
- EC 1.1.1.112
- EC 1.1.1.149,2-ALPHA-HSD
- EC 1.3.1.20
- HAKRC
- HAKRCDDH1aldo-keto reductase C
- HBAB
- hepatic dihydrodiol dehydrogenase
- High-affinity hepatic bile acid-binding protein
- Indanol dehydrogenase
- MBAB
- Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase
- type II 3-alpha-hydroxysteroid dehydrogenase
Background
The human aldo-keto reductases 1C1 and 1C3 (AKR1C1 and AKR1C3) have major roles in pre receptor regulation of progesterone action. They can both convert progesterone to the less potent efficiencies. AKR1C1 and AKR1C3 also act as 3-ketosteroid reductase, and as such they can convert the most potent androgen 5alpha-DHT into 3beta-andorstandiol, which is an estrogen receptor beta ligand, and into the inactive androgen 3alpha-androstnionl, respectively.