Aggrecan Antibody (MK-302)

Product: Ciprofibrate D8

Aggrecan Antibody (MK-302) Summary

Immunogen
Chondroitinase ABC-digested adult human aggrecan.
Marker
Chondrocyte Marker
Specificity
Reacts with a wide range of cartilage proteoglycans after either chondroitinase (ABC or AC) or testicular hyaluronidase digestion. Native proteoglycans do not react with MAB2035. Antibody binding to epitope is successfully inhibited by disaccharides of chondroitin-6-sulfate. Recognizes the chondroitin-6-sulfate stubs (preferentially in clusters) of high density cartilage proteoglycans of different species. No cross-reactivity with dermatan sulfate, keratan sulfate or chondroitin-4-sulfate.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ACAN
Purity
Unpurified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:5000
  • Radioimmunoassay 1:100-1:200
Application Notes
Chondroitinase ABC digestion prior to antibody reaction is required for antibody reactivity. {0.5U/mL in 100mM Tris-HCL, 30 minutes room temperature} A dramatic proteolytic digestion of the core protein (e.g. with papain or pronase) significantly reduces the antibody reactivity.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
No Preservative
Purity
Unpurified

Notes

Relevant Entrez Gene Numbers: NM_001135.2;NM_013227.2

Alternate Names for Aggrecan Antibody (MK-302)

  • ACAN
  • AGC1
  • AGC1SEDK
  • aggrecan core protein
  • Aggrecan
  • Cartilage-specific proteoglycan core protein
  • Chondroitin sulfate proteoglycan 1
  • Chondroitin sulfate proteoglycan core protein 1
  • CSPCP
  • CSPG1
  • CSPG1aggrecan 1
  • CSPGCP
  • large aggregating proteoglycan
  • MSK16
  • MSK16AGCAN
  • SEDK

PMID: 1335051