Aggrecan Antibody (MK-302) Summary
Immunogen |
Chondroitinase ABC-digested adult human aggrecan.
|
Marker |
Chondrocyte Marker
|
Specificity |
Reacts with a wide range of cartilage proteoglycans after either chondroitinase (ABC or AC) or testicular hyaluronidase digestion. Native proteoglycans do not react with MAB2035. Antibody binding to epitope is successfully inhibited by disaccharides of chondroitin-6-sulfate. Recognizes the chondroitin-6-sulfate stubs (preferentially in clusters) of high density cartilage proteoglycans of different species. No cross-reactivity with dermatan sulfate, keratan sulfate or chondroitin-4-sulfate.
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Isotype |
IgG1
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Clonality |
Monoclonal
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Host |
Mouse
|
Gene |
ACAN
|
Purity |
Unpurified
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Applications/Dilutions
Dilutions |
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Application Notes |
Chondroitinase ABC digestion prior to antibody reaction is required for antibody reactivity. {0.5U/mL in 100mM Tris-HCL, 30 minutes room temperature} A dramatic proteolytic digestion of the core protein (e.g. with papain or pronase) significantly reduces the antibody reactivity.
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Reactivity Notes
Packaging, Storage & Formulations
Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
|
Buffer |
Ascites
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Preservative |
No Preservative
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Purity |
Unpurified
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Notes
Alternate Names for Aggrecan Antibody (MK-302)
- ACAN
- AGC1
- AGC1SEDK
- aggrecan core protein
- Aggrecan
- Cartilage-specific proteoglycan core protein
- Chondroitin sulfate proteoglycan 1
- Chondroitin sulfate proteoglycan core protein 1
- CSPCP
- CSPG1
- CSPG1aggrecan 1
- CSPGCP
- large aggregating proteoglycan
- MSK16
- MSK16AGCAN
- SEDK