Product: ML241 (hydrochloride)
ATG7 Antibody Summary
| Immunogen |
Antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human APG7. The immunogen is located within amino acids 590 – 640 of APG7.
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| Specificity |
At least three isoforms of APG7 are known to exist; this antibody will detect all three isoforms. APG7 antibody is predicted not to cross-react with other ATG family proteins.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
ATG7
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
APG7 antibody can be used for the detection of APG7 by Western blot at 0.5 – 1 mg/ml.
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| Theoretical MW |
77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control Peptide |
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Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
PBS
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| Preservative |
0.02% Sodium Azide
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| Concentration |
1 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for ATG7 Antibody
- APG7 autophagy 7-like (S. cerevisiae)
- APG7
- APG7L
- APG7-LIKE
- ATG12-activating enzyme E1 ATG7
- ATG7 autophagy related 7 homolog (S. cerevisiae)
- ATG7
- Autophagy-related protein 7
- DKFZp434N0735
- GSA7
- hAGP7
- ubiquitin activating enzyme E1-like protein
- Ubiquitin-activating enzyme E1-like protein
- ubiquitin-like modifier-activating enzyme ATG7
Background
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog. In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death.