ARC/NOL3 Antibody Summary
| Immunogen |
A synthetic peptide made to residues 125-141 (PELQTLTRPGALRAPRR) of the human Nop30 protein.
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| Specificity |
This is specific for the human Nop30 protein (Gene names – ARC, NOP or NOL3).
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
NOL3
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
This antibody has been tested in Western blot, where a band at ~25 kDa is seen. No other applications have been tested. Western blot – Use at 1:500 to 1:1,000 dilution. *The investigator should determine the optimal dilution for each assay.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Theoretical MW |
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Positive Control |
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| Publications |
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Reactivity Notes
Reacts with residues 125-141 (PELQTLTRPGALRAPRR) of the human Nop30 protein. Reactivity to other species has not tested.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
PBS
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| Preservative |
0.02% Sodium Azide
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| Concentration |
1 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for ARC/NOL3 Antibody
- Apoptosis repressor with CARD
- ARC
- ARCFLJ35304
- CARD2
- Muscle-enriched cytoplasmic protein
- MYC
- MYP
- NOL3
- NOP30
- NOPnucleolar protein 3
- nucleolar protein 3 (apoptosis repressor with CARD domain)
- Nucleolar protein of 30 kDa
Background
Nop30 (nucleolar protein of 30 kDa) is predominantly expressed in muscle and is present in the nucleoplasm and concentrated in nucleoli. The predominant nucleolar localization of Nop30 points to a potential role in RNA processing in muscle nucleoli. A CARD domain containing protein, designated ARC for apoptosis repressor with CARD is related to Nop30 with a different carboxyl terminus.