APOBEC3G Antibody

Product: Ingenol-5,20-acetonide

APOBEC3G Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:MHNDTWVLLNQRRGFLCNQAPHKHGFLEGRHAELCFLDVIPFWKLDLDQDYRVTCFTSWSPCFSCAQEMAKFISKNKHVSLCIFTARIYDDQGRCQEGLRTLAEAGAKISIMTYSEFKHCWDTFVDHQGCPFQPWDGLDEHSQDLSGRL
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
APOBEC3G
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200 – 1:500
Application Notes
Immunohistochemistry retrieval recommended: HIER pH6,
Control Peptide
APOBEC3G Protein (NBP1-88592PEP)
Publications
Read Publications using NBP1-88592.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24025770)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for APOBEC3G Antibody

  • APOBEC-related cytidine deaminase
  • APOBEC-related protein 9
  • APOBEC-related protein
  • apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G
  • ARCD
  • ARP-9
  • bK150C2.7
  • CEM-15
  • CEM15ARP9
  • dJ494G10.1
  • DNA dC->dU-editing enzyme APOBEC-3G
  • EC 3.5.4
  • EC 3.5.4.-
  • EC 3.5.4.5
  • FLJ12740DNA dC->dU editing enzyme
  • MDS019
  • phorbolin-like protein MDS019

PMID: 9353357

APOBEC3G Antibody

Product: RI-3

APOBEC3G Antibody Summary

Immunogen
Antibody was raised against a synthetic peptide corresponding to 15 amino acids near the amino-terminus of human APOBEC3G. The immunogen is located within the first 50 amino acids of APOBEC3G.
Specificity
APOBEC3G antibody will also detect the APOBEC3F isoform.
Clonality
Polyclonal
Host
Rabbit
Gene
APOBEC3G
Purity
Immunogen affinity purified
Innovators Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Learn about the Innovators Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunofluorescence
Application Notes
APOBEC3G antibody can be used for detection of APOBEC3G by Western blot at 5 mg/ml.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for APOBEC3G Antibody

  • APOBEC-related cytidine deaminase
  • APOBEC-related protein 9
  • APOBEC-related protein
  • apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G
  • ARCD
  • ARP-9
  • bK150C2.7
  • CEM-15
  • CEM15ARP9
  • dJ494G10.1
  • DNA dC->dU-editing enzyme APOBEC-3G
  • EC 3.5.4
  • EC 3.5.4.-
  • EC 3.5.4.5
  • FLJ12740DNA dC->dU editing enzyme
  • MDS019
  • phorbolin-like protein MDS019

Background

The Apoliprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytidine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates. Similar to TRIM5alpha which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection as at least two isoforms, APOBEC3G and to a lesser extent APOBEC3F, can be incorporated HIV-1 virions and induce hypermutation in the newly synthesized viral DNA and thus destabilize the viral genome. This innate mechanism of retroviral resistance is counteracted by the HIV-1 Vif protein by inducing the ubiquitization and degradation of APOBEC3G; a single amino acid substitution (D128K) blocks APOBEC3G depletion without affecting its inhibitory activity.

PMID: 25657738