AICDA Antibody Summary
| Immunogen |
Antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human AID. The immunogen is located within the last 50 amino acids of AID.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
AICDA
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
This antibody can be used for detection of AID by Western blot at 1 – 2 mg/ml. Whole cell lysate from Ramos cells can be used as positive control. Anti-AID is human and mouse reactive.
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| Theoretical MW |
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control Peptide |
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Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
PBS
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| Preservative |
0.02% Sodium Azide
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| Concentration |
1 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for AICDA Antibody
- activation-induced cytidine deaminase
- AICDA
- AID
- AIDCytidine aminohydrolase
- Arp2
- CDA2
- HIGM2
- HIGM2EC 3.5.4.5
- integrated into Burkitts lymphoma cell line Ramos
Background
Activation-induced cytidine deaminase (AID) was initially discovered as a homolog of the apolipoprotein B RNA-editing cytidine deaminase 1 (APOBEC1) that showed cytidine deaminase properties in stimulated B cell lines. It is necessary for somatic hypermutation and class switch recombination in B cells, but inappropriate or dysregulated expression AID is often found in tumors and B cell neoplasms. Although it is structurally and functionally similar to the APOBEC proteins, it appears unlikely that AID deaminates dC to dU residues in HIV cDNA as does APOBEC3G.