ACAT1 Antibody

Product: Biperiden (Hydrochloride)

ACAT1 Antibody Summary

Immunogen
Peptide with sequence C-KRVDFSKVPKLKT corresponding to internal region according to NP_000010.1.
Epitope
C-KRVDFSKVPKLKT
Clonality
Polyclonal
Host
Goat
Gene
ACAT1
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

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Applications/Dilutions

Dilutions
  • Western Blot 0.01 – 0.03 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
IF: An anonymous customer has reported particulate cytoplasm staining in HeLa WB: Approx. 45 kDa band observed in human liver lysates and in rodent kidney and liver lysates (calculated MW of 45.2 kDa band according to NP_000010.1).

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Theoretical MW
45.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-51994.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ACAT1 Antibody

  • ACAT
  • acetoacetyl Coenzyme A thiolase
  • Acetoacetyl-CoA thiolase
  • acetyl-CoA acetyltransferase 1
  • acetyl-CoA acetyltransferase, mitochondrial
  • acetyl-Coenzyme A acetyltransferase 1
  • EC 2.3.1
  • EC 2.3.1.9
  • MAT
  • mitochondrial acetoacetyl-CoA thiolase
  • T2
  • THIL

Background

ACAT-1 is a ~50 kDa protein that catalyzes the formation of intracellular cholesterol esters in various tissues, such as adult human liver, adrenal glands, macrophages, and kidneys.

PMID: 8287965

ACAT1 Antibody

Product: GW 501516

ACAT1 Antibody Summary

Immunogen
Peptide with sequence C-DEEYKRVDFSKVPK corresponding to internal region according to NP_000010.1.
Clonality
Polyclonal
Host
Goat
Gene
ACAT1
Purity
Immunogen affinity purified
Innovators Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Learn about the Innovators Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.1 – 0.3 ug/ml
  • Peptide ELISA Detection limit 1:2000
Application Notes
IF: An anonymous customer has reported particulate cytoplasm staining in HeLa. WB: Approx. 45 kDa band observed in human, mouse and rat liver lysates (calculated MW of 45.2 kDa band according to human NP_000010.1, 44.8 kDa band according to mouse NP_659033.1 and 44.7 kDa band according to rat NP_058771.2).

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-46140.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ACAT1 Antibody

  • ACAT
  • acetoacetyl Coenzyme A thiolase
  • Acetoacetyl-CoA thiolase
  • acetyl-CoA acetyltransferase 1
  • acetyl-CoA acetyltransferase, mitochondrial
  • acetyl-Coenzyme A acetyltransferase 1
  • EC 2.3.1
  • EC 2.3.1.9
  • MAT
  • mitochondrial acetoacetyl-CoA thiolase
  • T2
  • THIL

Background

ACAT-1 is a ~50 kDa protein that catalyzes the formation of intracellular cholesterol esters in various tissues, such as adult human liver, adrenal glands, macrophages, and kidneys.

PMID: 25692473