ABCB5 Antibody

Product: Collagen proline hydroxylase inhibitor-1

ABCB5 Antibody Summary

Immunogen
Peptide with sequence C-QTQHRNTSKKAQ corresponding to internal region according to NP_848654.3, NP_001157413.1.
Epitope
C-QTQHRNTSKKAQ
Specificity
This antibody is expected to recognise both reported isoforms (NP_848654.3; NP_001157413.1)
Clonality
Polyclonal
Host
Goat
Gene
ABCB5
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.3 – 1 ug/ml
  • Flow Cytometry 1 ug
  • Peptide ELISA Detection limit 1:32000
Application Notes
FLOW successful usage on Hep3B cells is reported in scientific literature (PMID: 20682318). Data obtained from a previous batch (different goat). WB: Approx. 80 kDa band observed in lysates of A431(calculated MW of 89.8 kDa band according to NP_848654.3).

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Theoretical MW
90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
ABCB5 Lysate (NBL1-07174)
Publications
Read Publications using NB100-93365.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ABCB5 Antibody

  • ABCB5 P-gp
  • ABCB5
  • ABCB5alpha
  • ABCB5beta
  • ATP-binding cassette protein
  • ATP-binding cassette sub-family B member 5
  • ATP-binding cassette, sub-family B (MDR/TAP), member 5
  • EC 3.6.3
  • EC 3.6.3.44
  • EST422562
  • P-glycoprotein ABCB5

Background

ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM]

PMID: 1692975